Composite

Part:BBa_K4788008:Design

Designed by: Zhaocheng Li   Group: iGEM23_LZU-CHINA   (2023-10-11)


FadR-FadBA-FadO-mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 764
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1343
    Illegal AgeI site found at 1455
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to achieve oleic acid responsive function and further characterize oleic acid inducers, it is necessary to have a promoter in front of this part to express the FadR protein. Therefore, suitable promoters or plasmid skeletons should be selected in practical use.


Source

We synthesized it.

References

Ma Y, Zheng X, Lin Y, Zhang L, Yuan Y, Wang H, Winterburn J, Wu F, Wu Q, Ye JW, Chen GQ. Engineering an oleic acid-induced system for Halomonas, E. coli and Pseudomonas. Metab Eng. 2022 Jul;72:325-336. doi: 10.1016/j.ymben.2022.04.003. Epub 2022 May 2. PMID: 35513297.